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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
11/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOGONE, I. C. V. P.; FERREIRA, G. H.; GAVA, D.; SCHAEFER, R.; PAULA-LOPES, F. F. de; ROCHA, R. de A.; BARROS, F. R. O. de. |
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Afiliação: |
IZABEL C. V. P. COGONE, UTFPR; GLAUCIA H. FERREIRA, UNIFESP/Diadema; DANIELLE GAVA, CNPSA; REJANE SCHAEFER, CNPSA; FABÍOLA F. DE PAULA-LOPES, UNIFESP/Diadema; RAQUEL DE A. ROCHA, UTFPR; FLAVIA REGINA OLIVEIRA DE BARROS, UTFPR. |
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Título: |
Applicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 249, n. 119336, 2021. |
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DOI: |
https://doi.org/10.1016/j.saa.2020.119336 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications. MenosAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for bette... Mostrar Tudo |
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Palavras-Chave: |
Molecular fingerprint; PCV2; PPV; Reproductive failure; Viral diagnostic. |
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Thesagro: |
Diagnostico; Parvovirose; Sanidade Animal; Suíno. |
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Thesaurus Nal: |
Animal diseases; Porcine circovirus-2; Raman spectroscopy; Reproductive success; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02976naa a2200373 a 4500
001 2133480
005 2021-08-11
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.saa.2020.119336$2DOI
100 1 $aGOGONE, I. C. V. P.
245 $aApplicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection.$h[electronic resource]
260 $c2021
520 $aAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications.
650 $aAnimal diseases
650 $aPorcine circovirus-2
650 $aRaman spectroscopy
650 $aReproductive success
650 $aSwine
650 $aDiagnostico
650 $aParvovirose
650 $aSanidade Animal
650 $aSuíno
653 $aMolecular fingerprint
653 $aPCV2
653 $aPPV
653 $aReproductive failure
653 $aViral diagnostic
700 1 $aFERREIRA, G. H.
700 1 $aGAVA, D.
700 1 $aSCHAEFER, R.
700 1 $aPAULA-LOPES, F. F. de
700 1 $aROCHA, R. de A.
700 1 $aBARROS, F. R. O. de
773 $tSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy$gv. 249, n. 119336, 2021.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
04/01/2018 |
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Data da última atualização: |
09/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MALAQUIAS, J. B.; RAMALHO, F. de S.; DIAS, C. T. dos S.; BRUGGER, B. P.; LIRA, A. C. S.; WILCKEN, C. F.; PACHÚ, J. K. S.; ZANUNCIO, J. C. |
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Afiliação: |
JOSÉ B. MALAQUIAS, USP - ESALQ; FRANCISCO DE SOUSA RAMALHO, CNPA; CARLOS T. dos S. DIAS, USP - ESALQ; BRUNO P. BRUGGER, UFV; ALINE CRISTINA S. LIRA, CNPA; CARLOS F. WILCKEN, FACULDADE DE CIÊNCIAS AGRONÔMICAS, DEPARTAMENTO DE PROTEÇÃO VEGETAL, BOTUCATU; JÉSSICA K. S. PACHÚ, UFSCAR; JOSÉ C. ZANUNCIO, UFV. |
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Título: |
Multivariate approach to quantitative analysis of Aphis gossypii Glover (Hemiptera: Aphididae) and their natural enemy populations at different cotton spacings. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientific Reports, v. 7, 41740, 2017. |
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DOI: |
10.1038/srep41740 |
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Idioma: |
Inglês |
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Conteúdo: |
The relationship between pests and natural enemies using multivariate analysis on cotton in different spacing has not been documented yet. Using multivariate approaches is possible to optimize strategies to control Aphis gossypii at different crop spacings because the possibility of a better use of the aphid sampling strategies as well as the conservation and release of its natural enemies. The aims of the study were (i) to characterize the temporal abundance data of aphids and its natural enemies using principal components, (ii) to analyze the degree of correlation between the insects and between groups of variables (pests and natural enemies), (iii) to identify the main natural enemies responsible for regulating A. gossypii populations, and (iv) to investigate the similarities in arthropod occurrence patterns at different spacings of cotton crops over two seasons. High correlations in the occurrence of Scymnus rubicundus with aphids are shown through principal component analysis and through the important role the species plays in canonical correlation analysis. Clustering the presence of apterous aphids matches the pattern verified for Chrysoperla externa at the three different spacings between rows. Our results indicate that S. rubicundus is the main candidate to regulate the aphid populations in all spacings studied. |
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Palavras-Chave: |
Aphids. |
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Thesagro: |
Algodão; Aphis Gossypii; Inimigo natural; Praga. |
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Thesaurus NAL: |
Chrysoperla externa; Cotton; Natural enemies; Pests. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170271/1/Multivariate-approach-....pdf
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Marc: |
LEADER 02302naa a2200325 a 4500
001 2084168
005 2018-05-09
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1038/srep41740$2DOI
100 1 $aMALAQUIAS, J. B.
245 $aMultivariate approach to quantitative analysis of Aphis gossypii Glover (Hemiptera$bAphididae) and their natural enemy populations at different cotton spacings.$h[electronic resource]
260 $c2017
520 $aThe relationship between pests and natural enemies using multivariate analysis on cotton in different spacing has not been documented yet. Using multivariate approaches is possible to optimize strategies to control Aphis gossypii at different crop spacings because the possibility of a better use of the aphid sampling strategies as well as the conservation and release of its natural enemies. The aims of the study were (i) to characterize the temporal abundance data of aphids and its natural enemies using principal components, (ii) to analyze the degree of correlation between the insects and between groups of variables (pests and natural enemies), (iii) to identify the main natural enemies responsible for regulating A. gossypii populations, and (iv) to investigate the similarities in arthropod occurrence patterns at different spacings of cotton crops over two seasons. High correlations in the occurrence of Scymnus rubicundus with aphids are shown through principal component analysis and through the important role the species plays in canonical correlation analysis. Clustering the presence of apterous aphids matches the pattern verified for Chrysoperla externa at the three different spacings between rows. Our results indicate that S. rubicundus is the main candidate to regulate the aphid populations in all spacings studied.
650 $aChrysoperla externa
650 $aCotton
650 $aNatural enemies
650 $aPests
650 $aAlgodão
650 $aAphis Gossypii
650 $aInimigo natural
650 $aPraga
653 $aAphids
700 1 $aRAMALHO, F. de S.
700 1 $aDIAS, C. T. dos S.
700 1 $aBRUGGER, B. P.
700 1 $aLIRA, A. C. S.
700 1 $aWILCKEN, C. F.
700 1 $aPACHÚ, J. K. S.
700 1 $aZANUNCIO, J. C.
773 $tScientific Reports$gv. 7, 41740, 2017.
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Embrapa Algodão (CNPA) |
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